elisa-FLP100146 TLR9 Fig.1 Elisa 1

Human TLR9 full length protein-synthetic nanodisc

Price: 10 μg ￥6300.00 ;50 μg ￥30000.00 ; 100 μg ￥55000.00

SKU: FLP100146 Category: Tag:
Datasheet
MSDS
Product Data
Tag

C-Flag Tag
Target

Synonyms

CD289
Description

Human TLR9 full length protein-synthetic nanodisc
Delivery

In Stock
Uniprot ID

Q9NR96
Expression Host

HEK293
Protein Families

Druggable Genome, Transmembrane
Protein Pathways

Toll-like receptor signaling pathway
Molecular Weight

The human full length TLR9 protein has a MW of 115.9 kDa
Formulation & Reconstitution

Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments.
Storage & Shipping

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background

The protein is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
Usage

Research use only
Conjugate

Unconjugated
图片

elisa-FLP100146 TLR9 Fig.1 Elisa 1
Figure1. Elisa plates were pre-coated with Flag Tag TLR9-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with TLR9-Nanodisc is 2.467ng/ml.
page-FLP100146 TLR9 Fig.2 SDS PAGE 1
Figure2. Human TLR9-Nanodisc, Flag Tag on SDS-PAGE

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