| Tag | C-Flag Tag |
|---|---|
| Target | |
| Synonyms | NK1R, NKIR, SPR, TAC1R |
| Description | Human NK1R full length protein-synthetic nanodisc |
| Delivery | 6~8weeks |
| Uniprot ID | P25103 |
| Expression Host | HEK293 |
| Protein Families | GPCR,Transmembrane,Druggable Genome, |
| Protein Pathways | Peptide GPCRs,Glucocorticoid Signaling,cAMP and Ca2+ Signaling Pathway, |
| Molecular Weight | The human full length NK1R protein has a MW of 46.3kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene belongs to a gene family of tachykinin receptors. These tachykinin receptors are characterized by interactions with G proteins and contain seven hydrophobic transmembrane regions. This gene encodes the receptor for the tachykinin substance P, also referred to as neurokinin 1. The encoded protein is also involved in the mediation of phosphatidylinositol metabolism of substance P. [provided by RefSeq, Sep 2008] |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human NK1R full length protein-synthetic nanodisc
Price: 10 μg ￥6300.00 ;50 μg ￥30000.00 ; 100 μg ￥55000.00
Product Data
图片
Figure 1. Elisa plates were pre-coated with Flag Tag NK1R-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with NK1R-Nanodisc is 1.375ng/ml.
Figure 2. Human NK1R-Nanodisc, Flag Tag on SDS-PAGE
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