Target | |
---|---|
Synonyms | CMT1F;CMT2E;CMTDIG;NF-L;NF68;NFL;PPP1R110 |
Description | Recombinant Human NEFL(9-88) with C-terminal human Fc tag |
Delivery | In Stock |
Uniprot ID | P07196 |
Expression Host | HEK293 |
Tag | C-Human Fc Tag |
Molecular Characterization | NEFL(Tyr9-Thr88) hFc(Glu99-Ala330) |
Molecular Weight | The protein has a predicted molecular mass of 34.9 kDa after removal of the signal peptide. The apparent molecular mass of NEFL(9-88)-hFc is approximately 35-55 kDa due to glycosylation. |
Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008] |
Usage | Research use only |
Human NEFL (9-88) Protein, hFc Tag
Price: 10μg ¥818.00 ; 50μg ¥2999.00 ; 100 μg ¥4499.00
Product Data
图片
Figure 1. Human NEFL(9-88) Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human NEFL (9-88) Protein, hFc Tag (PME100654) can bind Anti-NEFL(9-88) antibody(DM198), Rabbit mAb DME100198 in a linear range of 0.128-80 ng/mL.
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