Target | |
---|---|
Synonyms | TNFSF14;CD258;HVEML;LIGHT;LTg |
Description | Recombinant Human LIGHT protein with N-terminal human Fc |
Delivery | In Stock |
Uniprot ID | O43557 |
Expression Host | HEK293 |
Tag | N-Human Fc Tag |
Molecular Characterization | hFc(Glu99-Ala330) LIGHT(Leu83-Val240) |
Molecular Weight | The protein has a predicted molecular mass of 43.4 kDa after removal of the signal peptide. |
Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. |
Usage | Research use only |
Human LIGHT Protein, hFc Tag
Price: 10μg ¥818.00 ; 50μg ¥2999.00 ; 100 μg ¥4499.00
Product Data
图片
Figure 1. Human LIGHT Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human HVEM, His tagged protein PME100273 can bind Human LIGHT, hFc tagged protein (PME100049) in a linear range of 0.32-40 ng/ml.
Figure 3. Flow cytometry analysis with 15 μg/mL Human LIGHT Protein, hFc tag (PME100049) on Expi293 cells transfected with human HVEM (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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