| Target | |
|---|---|
| Synonyms | IL2RA;CD25;p55;IL2-RA;IL-2-RA |
| Description | Recombinant human IL2RA Protein with C-terminal 6×His tag |
| Delivery | In Stock |
| Uniprot ID | P01589 |
| Expression Host | HEK293 |
| Tag | C-6×His Tag |
| Molecular Characterization | IL2RA(Glu22-Cys213) 6×His tag |
| Molecular Weight | The protein has a predicted molecular mass of 22.6 kDa after removal of the signal peptide.The apparent molecular mass of IL2RA-His is approximately 35-55 kDa due to glycosylation. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human IL2RA Protein, His Tag
Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00
Product Data
图片
Figure 1. Human IL2RA Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human IL2RA, His tagged protein (PME100098) can bind Anti-IL2RA Neutralizing antibody BME100043 in a linear range of 0.64-400 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL2RA Protein, His Tag (PME100098) can bind Human IL2 Protein, hFc Tag PME100300 in a linear range of 0.64-400 ng/mL.
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