| Tag | C-Flag Tag |
|---|---|
| Target | |
| Synonyms | ORXR1; OX1R; OXR1 |
| Description | Human HCRTR1 full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | O43613 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| Molecular Weight | The human full length HCRTR1 protein has a MW of 47.5 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human HCRTR1 full length protein-synthetic nanodisc
Price: 10 μg ￥6300.00 ;50 μg ￥30000.00 ; 100 μg ￥55000.00
Product Data
图片
Figure1. Elisa plates were pre-coated with Flag Tag HCRTR1-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with HCRTR1-Nanodisc is 4.139ng/ml.
Figure2. WB analysis of Human HCRTR1-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution
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