Human GPR75 full length protein-synthetic nanodisc

Name: Human GPR75 full length protein-synthetic nanodisc - 专注肿瘤免疫
SKU: FLP100031
Price: 8000.00 CNY
Availability: InStock

Price: 10 μg ￥6300.00 ;50 μg ￥30000.00 ; 100 μg ￥55000.00

SKU: FLP100031 Category: Full Length Transmembrane Proteins Tag: synthetic nanodisc

Datasheet

MSDS

Product Data

Tag	C-Flag Tag
Target	GPR75
Synonyms	GPRchr2; WI31133
Description	Human GPR75 full length protein-synthetic nanodisc
Delivery	In Stock
Uniprot ID	O95800
Expression Host	HEK293
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	N/A
Molecular Weight	The human full length GPR75 protein has a MW of 59.4 kDa
Formulation & Reconstitution	Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for
Storage & Shipping	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background	G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor.
Usage	Research use only
Conjugate	Unconjugated

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Figure1. Elisa plates were pre-coated with Flag Tag GPR75-Nanodisc (0.2μg/per well). Serial diluted anti-GPR75 monoclonal antibody (DMC100368) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-GPR75 monoclonal antibody binding with GPR75-Nanodisc is 303.6ng/ml.

FLP100031-GPR75-Fig.2-SDS-PAGE-1.jpg

Figure2. WB analysis of Human GPR75-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution

Human GPR75 full length protein-synthetic nanodisc

Product Data

图片

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