Tag | C-Flag&Strep Tag |
---|---|
Target | |
Synonyms | BMIQ10; GPR120; GPR129; GT01; O3FAR1; PGR4 |
Description | Human FFAR4-Strep full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | Q5NUL3 |
Expression Host | HEK293 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | N/A |
Molecular Weight | The human full length FFAR4-Strep protein has a MW of 42.2 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Lyophilized from PBS. Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | The protein is a G protein-coupled receptor (GPR) which belongs to the rhodopsin family of GPRs. The encoded protein functions as a receptor for free fatty acids, including omega-3, and participates in suppressing anti-inflammatory responses and insulin sensitizing. |
Usage | Research use only |
Conjugate | Unconjugated |
Human FFAR4-Strep full length protein-synthetic nanodisc
Price: 10 μg ¥5670.00 ;50 μg ¥27000.00 ; 100 μg ¥49500.00
Product Data
图片
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag FFAR4-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with FFAR4-Strep-nanodisc is 7.707ng/ml.
Figure 2. Human FFAR4-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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