Human CD27 Protein, mFc-His Tag

Name: Human CD27 Protein, mFc-His Tag - 专注肿瘤免疫
SKU: PME100013
Price: 4999.00 CNY
Availability: InStock

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

SKU: PME100013 Category: ECD Proteins

Datasheet

MSDS

Product Data

Target	CD27
Synonyms	CD27;TNFRSF7;S152;T14;Tp55
Description	Recombinant human CD27 protein with C-terminal mouse Fc and 6×His tag
Delivery	In Stock
Uniprot ID	P26842
Expression Host	HEK293
Tag	C-Mouse Fc and 6×His Tag
Molecular Characterization	CD27(Ala20-Arg191) mFc(Pro99-Lys330) 6×His
Molecular Weight	The protein has a predicted molecular mass of 65-72 kDa after removal of the signal peptide.
Purity	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution	Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Storage & Shipping	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background	The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Usage	Research use only
Conjugate	Unconjugated

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Figure 1. Human CD27 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, mFc-His tagged protein (PME100013) can bind Anti-CD27 Neutralizing antibody BME100018 in a linear range of 0.64-16.0 ng/ml.

Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD27, mFc-His tagged protein (PME100013) can bind Human CD70,hFc-His tagged protein PME100486 in a linear range of 3.2-80 ng/ml.

Figure 4. Flow cytometry analysis with 15 μg/mL Human CD27 Protein, mFc-His tag (PME100013) on Expi293 cells transfected with human CD70 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Human CD27 Protein, mFc-His Tag

Product Data

图片

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