| Clone ID | 2D3 |
|---|---|
| Target | |
| Synonyms | GPRC5D |
| Host Species | Rabbit |
| Description | Biotinylated Anti-GPRC5D antibody（DM61）, IgG Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | Q9NZD1 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA,IHC |
| Recommended Dilutions | ELISA 1/5000 IHC 1/100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at 2°C-8°C for 6 months |
| Background | The protein encoded by this gene is a member of the G protein-coupled receptor family. Recent studies demonstrate that GPRC5D is expressed on malignant bone marrow plasma cells, whereas normal tissue expression is limited to the hair follicle. It may represent a potential target for effector-cell–mediated therapy to treat plasma-cell disorders like MM. |
| Usage | Research use only |
| Conjugate | Biotinylated |
Biotinylated Anti-GPRC5D antibody(DM61), Rabbit mAb
Price: 10μg ￥1030.00 ; 50μg ￥3380.00 ; 100 μg ￥6080.00 ; 500 μg ￥19880.00
Product Data
图片
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human GPRC5D, mFc tagged protein can bind Biotinylated Anti-GPRC5D antibody(DM61) in a linear range of 0.488-2000 ng/ml
Figure 2. Immunohistochemical analysis of paraffin-embedded RPMI8226 transplanted tumor labeling biotinylated Anti-GPRC5D antibody (Cat#DME100061B) at 10μg/ml , followed by Streptavidin(HRP) ready to use. Membranous staining on RPMI8226 cells. Counter stained with Hematoxylin.Control: Used Anti-GPRC5D antibody (Cat#DME100061) instead of primary antibody, secondary antibody is Streptavidin (HRP) Ready to use.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Figure 3. DME100061B at 10µg/ml staining GPRC5D in in human tonsil tissue by IHC (SKU# DME100061B)
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