Anti-TNFRSF10B (tigatuzumab biosimilar) mAb

Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human TNFRSF10B, mFc tagged protein PME100465 can bind Anti-TNFRSF10B  Neutralizing antibody in a linear range of 3.2-80 ng/ml.

Figure 2. TNFRSF10B protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-TNFRSF10B (tigatuzumab) on Expi293 cells transfected with human TNFRSF10B(Blue histogram)or Expi293 transfected with irrelevant protein(Red histogram), and  Isotype antibody on Expi293 transfected with irrelevant protein(Orange histogram)

Figure 3. Flow cytometry analysis of antigen binding of anti-human TNFRSF10B mAb(BME100032).
(A) BME100032 does not bind to TT cells that do not express TNFRSF10B.
(B) A clear peak shift of BME100032 was seen compared to the control when incubated with TNFRSF10B-expressing SNU-5 cells, indicating strong binding of BME100032 to TNFRSF10B. Antibodies were incubated at 5 μg/mL.