| Clone ID | DM54 |
|---|---|
| Target | |
| Synonyms | SARS-CoV-2 RBD |
| Host Species | Rabbit |
| Description | Anti-SARS-CoV-2 RBD antibody(DM54); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P0DTC2 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | SARS-CoV-2 |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits; S1 and S2. S1 mainly contains a receptor binding domain (RBD); which accounts for recognizing the cell surface receptor; ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response. |
| Usage | Research use only；All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scrutinizing all patent application to ensure no IP infringement. |
| Conjugate | Unconjugated |
Anti-SARS-CoV-2 RBD antibody(DM54); Rabbit mAb
Price: 10μg ￥630.00 ; 50μg ￥1880.00 ; 100 μg ￥3380.00 ; 500 μg ￥11080.00
Product Data
图片
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Figure 1. Elisa plate pre-coated by 2 μg/ml (100μl/well) SARS-CoV-2 RBD protein can bind Rabbit Anti-SARS-CoV-2 RBD monoclonal antibody (clone:DM54) in a linear range of 0.1-100 ng/ml.
Figure 2. Competition flow cytometry assay demonstrating Rabbit anti-RBD monoclonal antibody (clone: DM54) blockade of SARS-CoV-2 (COVID-19) S protein RBD (1μg/ml, PME100497) binding to Expi 293 cell line transfected with human ACE2. IC50=816.4ng/ml. The Y-axis represents the geometric mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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