| Clone ID | DMC472 |
|---|---|
| Target | |
| Synonyms | EK8; HEK8; SEK; TYRO1 |
| Host Species | Rabbit |
| Description | Anti-EPHA4 antibody(DMC472); IgG1 Chimeric mAb |
| Delivery | In Stock |
| Uniprot ID | P54764 |
| IgG type | Rabbit/Human Fc chimeric IgG1 |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | Flow Cyt |
| Recommended Dilutions | Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events; particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq; Jan 2015] |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-EPHA4 antibody(DMC472); IgG1 Chimeric mAb
Price: 10μg ￥690.00 ; 50μg ￥2280.00 ; 100 μg ￥4080.00 ; 500 μg ￥13280.00
Product Data
图片
Figure 1. Flow cytometry analysis with Anti-EPHA4 (DMC472) on Expi293 cells transfected with human EPHA4 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. Flow cytometry analysis of antigen binding of anti-human EPHA4 mAb(DMC100472).
(A) DMC100472 does not bind to CHO-S cells that do not express EPHA4.
(B) A clear peak shift of DMC100472 was seen compared to the control when incubated with EPHA4-expressing MCF-7 cells, indicating strong binding of DMC100472 to EPHA4. Antibodies were incubated at 5 μg/mL.
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