| Clone ID | DM120 |
|---|---|
| Target | |
| Synonyms | CEACAM-5;CD66e;CEA;Meconium antigen 100 |
| Host Species | Rabbit |
| Description | Anti-CEACAM5 antibody(DM120); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P06731 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally; the encoded protein may regulate differentiation; apoptosis; and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. |
| Usage | Research use only；All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scrutinizing all patent application to ensure no IP infringement. |
| Conjugate | Unconjugated |
Anti-CEACAM5 antibody(DM120); Rabbit mAb
Price: 10μg ￥690.00 ; 50μg ￥2280.00 ; 100 μg ￥4080.00 ; 500 μg ￥13280.00
Product Data
图片
Figure 1. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human CEACAM5 protein, His tagged protein PME100071 can bind Rabbit anti-CEACAM5 monoclonal antibody (clone: DM120) in a linear range of 0.1-50 ng/ml.
Figure 2. Flow cytometry analysis with Anti-CEACAM5 (DM120) on Expi293 cells transfected with human CEACAM5(Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human CEACAM5 mAb(DME100120).
(A) DME100120 does not bind to 293T cells that do not express CEACAM5.
(B) A clear peak shift of DME100120 was seen compared to the control when incubated with CEACAM5-expressing HT55 cells, indicating strong binding of DME100120 to CEACAM5. Antibodies were incubated at 2 μg/mL.
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