Anti-CD99 antibody(DM203); Rabbit mAb

Name: Anti-CD99 antibody(DM203); Rabbit mAb - 专注肿瘤免疫
SKU: DME100203
Price: 438.00 CNY
Availability: InStock

Price: 10μg ￥690.00 ; 50μg ￥2280.00 ; 100 μg ￥4080.00 ; 500 μg ￥13280.00

SKU: DME100203 Category: Monoclonal Antibodies

Datasheet

MSDS

Product Data

Clone ID	DM203
Target	CD99
Synonyms	HBA71; MIC2; MIC2X; MIC2Y; MSK5X
Host Species	Rabbit
Description	Anti-CD99 antibody(DM203); Rabbit mAb
Delivery	In Stock
Uniprot ID	P14209
IgG type	Rabbit IgG
Clonality	Monoclonal
Reactivity	Human
Applications	ELISA; Flow Cyt; IHC
Recommended Dilutions	ELISA 1:5000-10000; Flow Cyt 1:100; IHC 1:100
Purification	Purified from cell culture supernatant by affinity chromatography
Formulation & Reconstitution	Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Storage & Shipping	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background	The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration; T-cell adhesion; ganglioside GM1 and transmembrane protein transport; and T-cell death by a caspase-independent pathway. In addition; the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus. [provided by RefSeq; Mar 2016]
Usage	Research use only；All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scrutinizing all patent application to ensure no IP infringement.
Conjugate	Unconjugated

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Figure 1. CD99 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CD99 (DM203) on Expi293 cells transfected with human CD99 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Figure 2. A. DME100203 at 10µg/ml staining CD99 in RPMI-8226 MM xenografts in NSG mice by IHC (SKU# DME100203);B: DME100203 at 10µg/ml staining CD99 in in human tonsil tissue by IHC (SKU# DME100203).

Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human CD99 mAb(DME100203).
(A) DME100203 does not bind to CHO-S cells that do not express CD99.
(B) A clear peak shift of DME100203 was seen compared to the control when incubated with CD99-expressing 8226 cells, indicating strong binding of DME100203 to CD99. Antibodies were incubated at 5 μg/mL.

Anti-CD99 antibody(DM203); Rabbit mAb

Product Data

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