Clone ID | 52A11 |
---|---|
Target | |
Synonyms | AGM6;B29;IGB |
Host Species | Rabbit |
Description | Anti-CD79B antibody(52A11); IgG1 Chimeric mAb |
Delivery | In Stock |
Uniprot ID | P40259 |
IgG type | Rabbit/Human Fc chimeric IgG1 |
Clonality | Monoclonal |
Reactivity | Human |
Applications | Flow Cyt; WB |
Recommended Dilutions | Flow Cyt 1:100; WB 1:1000 |
Purification | Purified from cell culture supernatant by affinity chromatography |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-beta protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Usage | Research use only;All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scrutinizing all patent application to ensure no IP infringement. |
Conjugate | Unconjugated |
Anti-CD79B antibody(52A11); IgG1 Chimeric mAb
Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00
Product Data
图片
Figure 1. Flow cytometry analysis with Anti-CD79B (52A11) mAb on Expi293 cells transfected with human CD79B (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Figure 2. Flow cytometry analysis of antigen binding of anti-human CD79B mAb(DMC101033).
(A) DMC101033 does not bind to 293T cells that do not express CD79B.
(B) A clear peak shift of DMC101033 was seen compared to the control when incubated with CD79B-expressing Raji cells, indicating strong binding of DMC101033 to CD79B. Antibodies were incubated at 5 μg/mL.
Figure 3.Anti-CD79B antibody (SKU# DMC101033) at 1/1000 dilution
Lane : RAJI (human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 26 kDa
Observed band size: 24 kDa
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