Anti-CD123 (talacotuzumab biosimilar) mAb

Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD123, hFc-His tagged protein (PME100003) can bind Anti-CD123 Neutralizing antibody in a linear range of 0.98-26.70 ng/ml. In order to specifically detect BME100003, mouse anti-human Fab-specific antibody was used as detection antibody.

Figure 2. HEK293 cell line transfected with irrelevant protein  (B) and human CD123  (A) were surface stained with anti-CD123 neutralizing antibody 1μg/ml (talacotuzumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Figure 3. Flow cytometry analysis of antigen binding of anti-human CD123 mAb(BME100003).
(A) A clear peak shift of BME100003 was seen compared to the control when incubated with CD123-expressing 8226 cells, indicating strong binding of BME100003 to CD123. Antibodies were incubated at 2 μg/mL.