Clone ID | DMC465 |
---|---|
Target | |
Synonyms | CD191; CKR-1; CKR1; CMKBR1; HM145; MIP1aR; SCYAR1 |
Host Species | Rabbit |
Description | Anti-CCR1 antibody(DMC465); IgG1 Chimeric mAb |
Delivery | In Stock |
Uniprot ID | P32246 |
IgG type | Rabbit/Human Fc chimeric IgG1 |
Clonality | Monoclonal |
Reactivity | Human |
Applications | Flow Cyt; WB |
Recommended Dilutions | Flow Cyt 1:100; WB 1:1000 |
Purification | Purified from cell culture supernatant by affinity chromatography |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | This gene encodes a member of the beta chemokine receptor family; which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha); regulated on activation normal T expressed and secreted protein (RANTES); monocyte chemoattractant protein 3 (MCP-3); and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response; and susceptibility to virus and parasite. This gene and other chemokine receptor genes; including CCR2; CCRL2; CCR3; CCR5 and CCXCR1; are found to form a gene cluster on chromosome 3p. [provided by RefSeq; Jul 2008] |
Usage | Research use only |
Anti-CCR1 antibody(DMC465); IgG1 Chimeric mAb
Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00
Product Data
图片
Figure 1. CCR1 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-CCR1 (DMC465) on Expi293 cells transfected with human CCR1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).
Figure 2.Anti-CCR1 antibody (SKU# DMC100465) at 1/1000 dilution
Lane : Hela, whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 41 kDa
Observed band size: 50 kDa
Figure 3. Flow cytometry analysis of antigen binding of anti-human CCR1 mAb(DMC100465).
(A) DMC100465 does not bind to CHO-S cells that do not express CCR1.
(B) A clear peak shift of DMC100465 was seen compared to the control when incubated with CCR1-expressing 8226 cells, indicating strong binding of DMC100465 to CCR1. Antibodies were incubated at 5 μg/ml.
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