| Tag | C-Flag Tag |
|---|---|
| Target | |
| Synonyms | IAP; MER6; OA3 |
| Description | Human CD47 full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | Q08722 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | ECM-receptor interaction |
| Molecular Weight | The human full length CD47 protein has a MW of 35.2 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C(Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | A membrane protein involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human CD47 full length protein-synthetic nanodisc
Price: 10 μg ￥6300.00 ;50 μg ￥30000.00 ; 100 μg ￥55000.00
Product Data
图片
Figure1. Elisa plates were pre-coated with Flag Tag CD47-Nanodisc (0.2μg/per well). Serial diluted anti-CD47 monoclonal antibody (BME100050) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CD47 monoclonal antibody binding with CD47-Nanodisc is 2.959ng/ml.
Figure2. Human CD47-Nanodisc, Flag Tag on SDS-PAGE
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