page-FLP100033 TRPA1 Fig.2 SDS PAGE 1

Human TRPA1 full length protein-synthetic nanodisc

Price: 10 μg ￥6300.00 ;50 μg ￥30000.00 ; 100 μg ￥55000.00

SKU: FLP100033 Category: Tag:
Datasheet
MSDS
Product Data
Tag

C-Flag Tag
Target

Synonyms

ANKTM1; FEPS; FEPS1
Description

Human TRPA1 full length protein-synthetic nanodisc
Delivery

In Stock
Uniprot ID

O75762
Expression Host

HEK293
Protein Families

Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
Protein Pathways

N/A
Molecular Weight

The human full length TRPA1 protein has a MW of 127.5 kDa
Formulation & Reconstitution

Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments.
Storage & Shipping

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background

The structure of the protein is highly related to both the protein ankyrin and transmembrane proteins. This protein is activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner, whereas mechanisms of non-electrophilic ligands are not well determined. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
Usage

Research use only
Conjugate

Unconjugated
图片

elisa-FLP100033 TRPA1 Fig.1 Elisa 1
Figure1. Elisa plates were pre-coated with Flag Tag TRPA1-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with TRPA1-Nanodisc is 7.433ng/ml.
page-FLP100033 TRPA1 Fig.2 SDS PAGE 1
Figure2. Human TRPA1-Nanodisc, Flag Tag on SDS-PAGE

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