Clone ID | DM80 |
---|---|
Target | |
Synonyms | AITR; GITR; TNFRSF18; CD357 |
Host Species | Rabbit |
Description | Anti-GITR antibody(DM80); Rabbit mAb |
Delivery | In Stock |
Uniprot ID | Q9Y5U5 |
IgG type | Rabbit IgG |
Clonality | Monoclonal |
Reactivity | Human |
Applications | ELISA; Flow Cyt |
Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
Purification | Purified from cell culture supernatant by affinity chromatography |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation; and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25( )CD4( ) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
Usage | Research use only |
Anti-GITR antibody(DM80); Rabbit mAb
Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00
Product Data
图片
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human GITR protein, hFc-His tagged protein PME100018 can bind Rabbit anti-GITR monoclonal antibody (clone: DM80) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human GITR (B) were surface stained with Rabbit anti-GITR monoclonal antibody 1μg/ml (clone: DM80) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-GITR monoclonal antibody (clone: DM80) on PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Affinity ranking of different Rabbit anti-GITR mAb clones by titration of different concentration onto PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 5. Flow cytometry analysis of antigen binding of rabbit anti-human GITR mAb(DME100080).
(A) DME100080 does not bind to 293T cells that do not express GITR.
(B) A clear peak shift of DME100080 was seen compared to the control when incubated with GITR-expressing 8226 cells, indicating strong binding of DME100080 to GITR. Antibodies were incubated at 2 μg/mL.
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