Human CD30 Protein, His tag

Name: Human CD30 Protein, His tag - 专注肿瘤免疫
SKU: PME100481
Price: 4999.00 CNY
Availability: InStock

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

SKU: PME100481 Category: ECD Proteins

Datasheet

MSDS

Product Data

Target	CD30
Synonyms	TNFRSF8;CD30;D1S166E;Ki-1
Description	Recombinant human CD30 protein with C-terminal 6×His tag
Delivery	In Stock
Uniprot ID	P28908
Expression Host	HEK293
Tag	C-6×His Tag
Molecular Characterization	CD30(Phe19-Lys379) 6×His tag
Molecular Weight	The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide. The apparent molecular mass of CD30-His is approximately 55-70 kDa due to glycosylation.
Purity	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution	Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Storage & Shipping	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background	The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Usage	Research use only
Conjugate	Unconjugated

图片

sp-PME100481 CD30 Fig.1 SDS PAGE 1

Figure 1. Human CD30 Protein, His Tag on SDS-PAGE under reducing condition.

elisa-PME100481 CD30 His ELISA Fig2

Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD30, His tagged protein (PME100481) can bind Anti-CD30 Neutralizing antibody (BME100017) in a linear range of 0.13-80.0 ng/ml.

elisa-PME100481 CD30 His ELISA Fig3

Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD30, His tagged protein (PME100481) can bind Human CD30 Ligand,mFc-His tagged protein (PME100041) in a linear range of 2.56-320 ng/ml.

fc-PME100481 CD30 His flow转染CD30L Fig4

Figure 4. Flow cytometry analysis with 1 μg/mL Human CD30 Protein, His tag (PME100481) on Expi293 cells transfected with human CD30L (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Human CD30 Protein, His tag

Product Data

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