SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag

Name: SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag - 专注肿瘤免疫
SKU: PME100460
Price: 4299.00 CNY
Availability: InStock

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

SKU: PME100460 Category: ECD Proteins

Datasheet

MSDS

Product Data

Target	S protein RBD
Synonyms	S protein RBD;Spike glycoprotein Receptor-binding domain;S glycoprotein RBD;Spike protein RBD;COVID-19
Description	Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD with C-terminal mouse Fc and 6×His tag
Delivery	In Stock
Uniprot ID	P0DTC2
Expression Host	HEK293
Tag	C-Mouse Fc and 6×His Tag
Molecular Characterization	S protein RBD(Arg319-Phe541) mFc(Pro99-Lys330) 6×His tag
Molecular Weight	The protein has a predicted molecular mass of 52.2 kDa after removal of the signal peptide.
Purity	The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution	Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Storage & Shipping	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background	SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.
Usage	Research use only
Conjugate	Unconjugated

图片

Figure 1. SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His tagged protein (PME100460) can bind Anti-SARS-CoV-2 S protein RBD Neutralizing Antibody (A neutralizing monoclonal antibody clone currently under clinical investigation from collaboration company) in a linear range of 0.24-9.141 ng/ml.

Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PME100460) can bind Anti-SARS-CoV-2 Neutralizing antibody CB6 BME100011 in a linear range of 0.24-15.62 ng/ml.

Figure 4. ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PME100460) can bind Human ACE2, hFc Tagged protein PME100073 in a linear range of 0.488-49.83 ng/ml.

SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag

Product Data

图片

相关产品

Login