| Tag | N-MBP Tag, C-Flag Tag |
|---|---|
| Target | |
| Synonyms | GPRchr2; WI31133 |
| Description | Human MBP-GPR75 full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | O95800 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | N/A |
| Molecular Weight | The human full length MBP-GPR75 protein has a MW of 99.4 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Lyophilized from PBS. Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human MBP-GPR75 full length protein-synthetic nanodisc
Price: 10 μg ￥6300.00 ; 50 μg ￥30000.00 ; 100 μg ￥55000.00
Product Data
图片
Figure 1. Elisa plates were pre-coated with N-MBP Tag, C-Flag Tag MBP-GPR75-Nanodisc (0.2μg/per well). Serial diluted anti-GPR75 monoclonal antibody (DMC100368) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-GPR75 monoclonal antibody binding with MBP-GPR75-Nanodisc is 21.90ng/ml.
Figure 2. Human MBP-GPR75-Nanodisc with N-MBP Tag, C-Flag Tag on SDS-PAGE
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