产品针对靶点:
2B4 | 4-1BB | 4-1BB-L | SIRPα |
B7-H3 | BCMA | CD123 | CD48 |
CD138 | CD22 | CD27 | GITR Ligand |
CD28 | CD38 | CD47 | CS1 |
GPRC5D | CTLA-4 | MSLN | TROP2 |
GITR | TIM3 | B7-2 | PSCA |
NKp30 | BTN3A1 | DNAM1 | B7-H2 |
CD40 |
与其他动物相比,兔拥有独特的B细胞发育过程,并且比啮齿动物与人类的亲缘性更远。除此之外,兔B细胞具有双重亲和力成熟机制,包括基因转换和SHM(体细胞超突变);同时,兔IgG的蛋白结构与鼠IgG不同,体现在其只拥有一个亚型IgG,兔轻链上特有的额外二硫键,以及CDR3的长度和序列差异很大等方面。这些不同之处使得兔单抗具有更高的亲和力和特异性,也更有可能筛选出具有差异化的抗体。
缔码生物创新性的兔重组单克隆抗体平台,聚焦肿瘤免疫靶点,使用功能活性充分验证的重组蛋白作为抗原免疫兔子,直接从阳性兔B细胞中克隆IgG,重组表达进行流式验证。这一技术不仅大大缩短了研发的时间,也让我们能够直接获得IgG的基因序列信息,而无需担心杂交瘤细胞丢失。
2B4 | 4-1BB | 4-1BB-L | SIRPα |
B7-H3 | BCMA | CD123 | CD48 |
CD138 | CD22 | CD27 | GITR Ligand |
CD28 | CD38 | CD47 | CS1 |
GPRC5D | CTLA-4 | MSLN | TROP2 |
GITR | TIM3 | B7-2 | PSCA |
NKp30 | BTN3A1 | DNAM1 | B7-H2 |
CD40 |
图 1. A) Flow cytometry data of serially titrated Rabbit anti-SIRPα monoclonal antibody (clone: DM8). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. B) Competition assay demonstrating DM8 blockade of CD47 binding to THP-1 cells. IC50=30.88ng/ml.
图 2. Affinity ranking of different Rabbit anti-CS1 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.