Anti-CD70 (vorsetuzumab biosimilar) mAb

Figure 1 . ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD70, mFc-His tagged protein PME100042 can bind Anti-CD70 Neutralizing antibody  in a linear range of 3.91-139.9 ng/ml.

Figure 2. HEK293 cell line transfected with irrelevant protein  (B) and human CD70  (A) were surface stained with anti-CD70 neutralizing antibody 1μg/ml (vorsetuzumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Figure 3. Flow cytometry analysis of antigen binding of anti-human CD70 mAb(BME100005).
(A) A clear peak shift of BME100005 was seen compared to the control when incubated with CD70-expressing THP1 cells, indicating strong binding of BME100005 to CD70. Antibodies were incubated at 2 μg/mL.