Clone ID | DM77 |
---|---|
Target | |
Synonyms | CD33;SIGLEC3;gp67 |
Host Species | Rabbit |
Description | Biotinylated Anti-CD33 antibody(DM77); Rabbit mAb |
Delivery | 2-3 weeks |
Uniprot ID | P20138 |
IgG type | Rabbit IgG |
Clonality | Monoclonal |
Reactivity | Human |
Applications | ELISA; Flow Cyt |
Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
Purification | Purified from cell culture supernatant by affinity chromatography |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins; two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6:SHP-1 and PTPN11:SHP-2. In turn; these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase:PI3K. |
Usage | Research use only |
Biotinylated Anti-CD33 antibody(DM77); Rabbit mAb
Price: 10μg ¥1030.00 ; 50μg ¥3380.00 ; 100 μg ¥6080.00 ; 500 μg ¥19880.00
Product Data
图片
相关产品
ECD Proteins
SKU: PME100693 Target: CD33
Price: 10μg ¥636.00 ; 50μg ¥2333.00 ; 100 μg ¥3499.00
Biosimilar reference antibodies
SKU: BME100015B Target: CD33
Application: Flow Cyt
Price: 50 μg ¥890.00 ; 100 μg ¥1699.00
ECD Proteins
SKU: PME-M100015 Target: CD33
Price: 10μg ¥673.00 ; 50μg ¥2466.00 ; 100 μg ¥3699.00
DiSliceX
SKU: SLI100011 Target: B7H5, BST1, CCR2, CD33, CD70, CD93, CHI3L1, TREM2
Application: IHC
Price: 2 slides ¥899.00
Biosimilar reference antibodies
SKU: BME100015 Target: CD33
Application: Flow Cyt
Price: 50 μg ¥800.00 ; 100 μg ¥1500.00