| Clone ID | DM94 |
|---|---|
| Target | |
| Synonyms | BTN3A1; BTF5; CD277; BTN3.1; BT3.1 |
| Host Species | Rabbit |
| Description | Anti-BTN3A1 antibody(DM94); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | O00481 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt; WB |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100; WB 1:1000 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g.; BTN2A1; MIM 613590) and BTN3 (e.g.; BNT3A1) genes; which have undergone tandem duplication; resulting in 3 copies of each. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-BTN3A1 antibody(DM94); Rabbit mAb
Price: 10μg ￥690.00 ; 50μg ￥2280.00 ; 100 μg ￥4080.00 ; 500 μg ￥13280.00
Product Data
图片
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BTN3A1 protein, mFc-His tagged protein PME100056 can bind Rabbit anti-BTN3A1 monoclonal antibody ( clone: DM94) in a linear range of 0.64-80 ng/ml.
Figure 2. Flow cytometry analysis with Anti-BTN3A1 (DM94) on Expi293 cells transfected with human BTN3A1 (Red histogram) or Expi293 transfected with irrelevant protein (Blue histogram).
Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human BTN3A1 mAb(DME100094).
(A) DME100094 does not bind to MCF-7 cells that do not express BTN3A1.
(B) A clear peak shift of DME100094 was seen compared to the control when incubated with BTN3A1-expressing 8226 cells, indicating strong binding of DME100094 to BTN3A1. Antibodies were incubated at 2 μg/mL.
Figure 4.Anti-BTN3A1 antibody (SKU# DME100094) at 1/1000 dilution
Lane : RPMI8226, whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution
Predicted band size: 33 kDa
Observed band size: 35，54，68 kDa
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