SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag
|Package||10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00|
|Description||Recombinant SARS-CoV-2 (2019-nCoV) S protein RBD with C-terminal mouse Fc and 6×His tag|
|Synonyms||S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD, COVID-19|
|Tag||C-Mouse Fc and 6×His tag|
|Molecular Weight||The protein has a predicted molecular mass of 52.2 kDa after removal of the signal peptide.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Storage||Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature.|
|Usage||Research use only|
Figure 1. SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His tagged protein (PME100460) can bind Anti-SARS-CoV-2 S protein RBD Neutralizing Antibody (A neutralizing monoclonal antibody clone currently under clinical investigation from collaboration company) in a linear range of 0.24-9.141 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human S-RBD, mFc-His tagged protein (PME100460) can bind Anti-SARS-CoV-2 Neutralizing antibody(CB6) (BME100011) in a linear range of 0.24-15.62 ng/ml.
Figure 4. ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PME100460) can bind Human ACE2, hFc Tagged protein（PME100073） in a linear range of 0.488-49.83 ng/ml.
|Background||SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.|