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Human CD63 full length protein-MNP

产品概述
Catalog Number FLP100034
package 50 μg ¥8000.00 ; 100 μg ¥10800.00
Description Human CD63 full length protein membrane nanoparticles (MNPs)
Expression Host HEK293
Symbol CD63
ORF Size 714 bp
Formulation Supplied in 1xPBS (pH 7.4)
Storage The product should be stored at -80°C. Please do not repeated freeze-thaw cycles.
ACCN NM_001780
Protein Families Druggable Genome, Transmembrane
Protein Pathways Lysosome
MW The human full length CD63 protein has a MW of 25.6 kDa
Protein Summary The protein is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms.
UniProt ID P08962
Delivery In Stock
Images

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Figure1. Elisa plates were pre-coated with 0.5μg/per well purified human CD63 full length membrane nanoparticles. Serial diluted anti-CD63 monoclonal antibody (DMC100425) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CD63 monoclonal antibody binding with CD63 full length membrane nanoparticles is 61.65ng/ml.

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Figure2. FACS analysis of CD63 MNPs
A. Negative Control 1: CD63 full length membrane nanoparticles samples were stained only with Goat anti-human lgG 488 secondary antibody.
B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-CD63 antibody (DMC100425) at 2μg/mL, followed by Goat anti-human IgG 488 secondary antibody.
C. Negative Control 3: CD63 full length membrane nanoparticles samples were stained with anti-GPRC5D antibody (an irrelevant antibody) at 2μg/mL, followed by Goat anti-human IgG 488 secondary antibody.
D. CD63 full length membrane nanoparticles samples were stained with anti-CD63 antibody (DMC100425) at 2μg/mL, followed by Goat anti-human IgG 488 secondary antibody.

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